Cell

Part:BBa_K566032:Design

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-27)

E. coli MXRedGreen


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 4101
    Illegal SpeI site found at 2653
    Illegal SpeI site found at 4196
    Illegal PstI site found at 5440
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 4101
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 963
    Illegal NheI site found at 986
    Illegal NheI site found at 1895
    Illegal NheI site found at 1918
    Illegal NheI site found at 2809
    Illegal SpeI site found at 2653
    Illegal SpeI site found at 4196
    Illegal PstI site found at 5440
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 4101
    Illegal BamHI site found at 2354
    Illegal XhoI site found at 5118
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 4101
    Illegal SpeI site found at 2653
    Illegal SpeI site found at 4196
    Illegal PstI site found at 5440
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 4101
    Illegal SpeI site found at 2653
    Illegal SpeI site found at 4196
    Illegal PstI site found at 5440
    Illegal NgoMIV site found at 386
    Illegal AgeI site found at 4136
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

UANLBricks assambly


Source

E. coli strain JT2, plasmids of Dr. Jeff Tabor

References

1. Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in Escherichia coli. J Mol Biol 405:315-324.